Journal: EMBO Molecular Medicine

Article Title: The HSP40 chaperone Ydj1 drives amyloid beta 42 toxicity

doi: 10.15252/emmm.202113952

Figure Lengend Snippet: A Pearson’s correlation coefficient and Manders’ co‐localization Coefficients of endoplasmatic reticulum protein KDEL and Abeta analyzed on confocal images of Kenyon cells in 15‐day‐old male fly brains immunostained with Abeta‐specific antibody (Abeta) 6E10 and KDEL‐specific antibody in Droj2 knockdown flies ( Droj2 +/− ) and corresponding isogenic w 1118 wild‐type flies ( Droj2 +/+ ) with expression of human Abeta42 (UAS‐A42). Dot plots show all data points along with the mean (bar) ± SD n = 10. Unpaired, two‐tailed t ‐test. B Representative confocal and gSTED deconvolved (decon) images of Kenyon cells in 15‐day‐old male fly brains immunostained with Abeta‐specific antibody (Abeta) 6E10 (magenta) and endoplasmic reticulum protein KDEL‐specific antibody (KDEL, green) of Droj2 knockdown flies ( Droj2 +/− ) and corresponding isogenic w 1118 wild‐type flies ( Droj2 +/+ ) expressing human Abeta42 (UAS‐A42). C Representative confocal and gSTED deconvolved (decon) microscopy of Kenyon cells in 15‐day‐old male fly brains immunostained with Abeta‐specific antibody (Abeta) 6E10 (magenta) and mitochondrial marker ATP5A‐specific antibody (ATP5A, green) of w 1118 wild‐type flies ( Droj2 +/+ ) expressing human Abeta42 (UAS‐A42). D–H Counts of mitochondria (D), mitochondria average size (E), mitochondria coverage area (F), Feret diameter (G), and min Feret diameter (H) of ATP5A‐stained mitochondria from fly brain gSTED deconvolved images representatively shown in Fig from 9 to 10 brains of w 1118 wild‐type ( Droj2 +/+ ) and knockdown ( Droj2 +/− ) male flies expressing human Abeta42 (UAS‐A42). Dot plots show all data points along with the mean (bar) ± SD n = 9–10. Unpaired, two‐tailed t ‐test or Mann–Whitney test.

Article Snippet: The following primary antibodies were used: mouse monoclonal IgG1 κ anti‐Aß antibody (clone 6E10; Biolegend, 803001; 1:200 for confocal, 1:400 for gSTED), mouse IgG2b anti‐ATP5A (clone 15H4C4, Abcam, ab14748, 1:100 for gSTED), and mouse IgG2a anti‐KDEL (clone 10C3, Enzo Lifesciences, ADI‐SPA‐827, 1:100 for gSTED).

Techniques: Expressing, Two Tailed Test, Microscopy, Marker, Staining, MANN-WHITNEY

Journal: Nutrients

Article Title: Anti-Inflammatory Activity of Isomaltodextrin in a C57BL/6NCrl Mouse Model with Lipopolysaccharide-Induced Low-Grade Chronic Inflammation

doi: 10.3390/nu11112791

Figure Lengend Snippet: Effect of IMD on PPAR-γ and IRS-1expression in white adipose tissue of mice as determined by Western blot. Results are expressed as mean ± SEM for n = 4 samples per group. Differences in means were considered statistically significant for * p < 0.05 or ** p < 0.01.

Article Snippet: The membranes were washed 5 times, for 5 minutes each, and incubated with the secondary antibody diluted in 3% BSA in TBST (1:2000 ( v / v ) diluted anti-mouse for PPAR-γ and TLR-4, or 1:1000 ( v / v ) diluted anti-rabbit for IRS-1 (Promega, Madison, WI)) for one hour at room temperature with gentle shaking.

Techniques: Western Blot